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Title:

Evaluation of fluorescence images acquired from oligonucleotide libraries

Group publication title:

Optica Applicata

Creator:

Cysewska-Sobusiak, Anna ; Bołtrukiewicz, Michał ; Parzych, Joanna

Subject and Keywords:

optyka ; CCD matrix ; DNA ; fluorescence ; image analysis

Description:

Optica Applicata, Vol. 38, 2008, nr 2, s. 375-386

Publisher:

Oficyna Wydawnicza Politechniki Wrocławskiej

Place of publication:

Wrocław

Contributor:

Gaj, Miron. Redakcja ; Urbańczyk, Wacław. Redakcja

Date:

2008

Resource Type:

artykuł

Source:

<sygn. PWr A3481II> ; click here to follow the link ; click here to follow the link

Language:

eng

Relation:

Optica Applicata ; Optica Applicata, Vol. 38, 2008 ; Optica Applicata, Vol. 38, 2008, nr 2 ; Politechnika Wrocławska. Wydział Podstawowych Problemów Techniki

Location:

Politechnika Wrocławska

Abstrakt:

By using a given DNA sequence, labeled with an organic dye, fluorescence may be induced as the effect of the hybridization of the sequence with complementary oligonucleotides located in a so-called library. The analysis consists in the detection of lighting points, in which the hybridization occurred. Each image of the created matrix corresponds to the optical response of a given oligonucleotide included in the investigated sequence spectrum. A novel approach to modeling of images obtained from a virtual oligonucleotide library and detected by a CCD camera is presented. Tests to determine optimum algorithms of the oligonucleotide library detection were carried out in the form of a computer simulation. Analyzed recorded images do not represent the actual illuminated oligonucleotide library, but were obtained by simulation in an identical way and by means of the same set of instruments. As a result, it was proven how to obtain correct detection depending on a mutual location of a given oligonucleotide library and the CCD matrix.

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